A paper on the detection of ultra-trace levels of Alzheimer’s disease-related substances in blood was published in Analytical Chemistry, one of the world’s most authoritative journals in the field of analytical chemistry, including mass spectrometry.
Previously, measurements such as ELISA measurements of the amount of Amyloid β [1-40], [1-42] in spinal fluid were used as an indicator for the progression of Alzheimer’s disease. This paper however, reports the detection of Amyloid β [1-28] to sub-fmol level in blood containing an extremely wide abundance of compounds using a modified version of the previously developed Fishing method (immunobeads-mass spectrometry).
Naoki Kaneko1, Takayuki Yoshimori1, Rie Yamamoto1, Daniel J. Capon2, Takashi Shimada3, Taka-Aki Sato1,3, and Koichi Tanaka1
“Multi Epitope-Targeting Immunoprecipitation Using F(ab’) Fragments with High Affinity and Specificity for the Enhanced Detection of a Peptide with Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry”, Analytical Chemistry., 2013, Vol. 85 No.6, pp3152-3159
1 Koichi Tanaka Laboratory of Advanced Science and Technology, Shimadzu Corporation
2 Blood Systems Research Institute
3 Life Science Research Center, Technology Research Laboratory, Shimadzu Corporation
Presentation materials (Link to overview of the paper published in Analytical Chemistry)
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Newly-arrived related information:
Nov. 7, 2011: [Materials Provided] The paper “Immunobeads-Mass Spectrometry” published.
