Feb. 7, 2012: [Materials Provided] Paper on “MS Cysteine High Sensitivity Labeling Methods” published.

 A paper by the Koichi Tanaka Laboratory of Advanced Science and Technology has been published in “Analytical Biochemistry,” a globally prominent academic journal primarily in the field of bio-related analytical chemistry.

 With conventional enzyme digestion for protein identification, carbamide methylation (labeling) (conventional method) after disulfide bond reduction has been the standard. In this paper, results show that it is possible to achieve up to a 200 fold increase in sensitivity over the conventional method through the development of new labeling agents.

 A number of MS data sets are introduced in details in the <Supplementary content>.

Takashi Shimada1,2, Hiroki Kuyama1, Taka-Aki Sato1,2, Koichi Tanaka1
“Development of iodoacetic acid-based cysteine mass tags: Detection enhancement for cysteine-containing peptide by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry”, Analytical Biochemistry, 2012, Issue 2, pp785-787
1 SHIMADZU Corporation, Koichi Tanaka Laboratory of Advanced Science and Technology
2 SHIMADZU Corporation, Life Science Research Center

Presentation materials (Link to ScienceDirect)